Confocal imaging of U-2 OS cells using GCSH Rabbit mAb (STJ11107342, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with รŽยฑ-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-GCSH antibody (49-173 aa) [SRM5429] (STJ11107342)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11107342
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General Information

Short DescriptionRabbit monoclonal GCSH (49-173 aa) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIF/ICC/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDSRM5429
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeIF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolGCSH
Gene ID2653
Uniprot IDGCSH_HUMAN
Immunogen Region49-173 aa
Immunogen SequenceSVRKFTEKHEWVTTENGIGT VGISNFAQEALGDVVYCSLP EVGTKLNKQDEFGALESVKA ASELYSPLSGEVTEINEALA ENPGLVNKSCYEDGWLIKMT LSNPSELDELMSEEAYEKYI KSIEE
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 49-173 of human GCSH (NP_004474.2).

Additional Info

Function The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST). Has a pivotal role in the lipoylation of enzymes involved in cellular energetics such as the mitochondrial dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT), and the mitochondrial dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (DLST).
Protein Name Glycine Cleavage System H Protein - Mitochondrial
Lipoic Acid-Containing Protein
Database Links Reactome: R-HSA-6783984
Reactome: R-HSA-9857492
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Glycine Cleavage System H Protein - Mitochondrial antibody
Anti-Lipoic Acid-Containing Protein antibody
Anti-GCSH antibody

Information sourced from Uniprot.org

Citations

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