![Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T (KO) cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ115042) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94082/375468/STJ115042_1__34610.1713142235.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T (KO) cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ115042) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T (KO) cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ115042) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/94082/375468/STJ115042_1__34610.1713142235.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T (KO) cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ115042) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
Anti-GCN1L1 antibody (2462-2671) (STJ115042)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GCN1L1 (2462-2671) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GCN1 |
| Gene ID | 10985 |
| Uniprot ID | GCN1_HUMAN |
| Immunogen Region | 2462-2671 |
| Immunogen Sequence | LSAVLQQCLLADVSGIDWMV RHGRSLALSVAVNVAPGRLC AGRYSSDVQEMILSSATADR IPIAVSGVRGMGFLMRHHIE TGGGQLPAKLSSLFVKCLQN PSSDIRLVAEKMIWWANKDP LPPLDPQAIKPILKALLDNT KDKNTVVRAYSDQAIVNLLK MRQGEEVFQSLSKILDVASL EVLNEVNRRSLKKLASQADS TEQVDDTILT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 2462-2671 of human GCN1L1 (NP_006827.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Expressed in skeletal muscules, ovary and testis. |
| Function | Ribosome collision sensor that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. Directly binds to the ribosome and acts as a sentinel for colliding ribosomes: activated following ribosome stalling and promotes recruitment of RNF14, which directly ubiquitinates EEF1A1/eEF1A, leading to its degradation. In addition to EEF1A1/eEF1A, the RNF14-RNF25 translation quality control pathway mediates degradation of ETF1/eRF1 and ubiquitination of ribosomal protein. GCN1 also acts as a positive activator of the integrated stress response (ISR) by mediating activation of EIF2AK4/GCN2 in response to amino acid starvation. Interaction with EIF2AK4/GCN2 on translating ribosomes stimulates EIF2AK4/GCN2 kinase activity, leading to phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1). EIF2S1/eIF-2-alpha phosphorylation converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. |
| Protein Name | Stalled Ribosome Sensor Gcn1Gcn1 Eif-2-Alpha Kinase Activator HomologGcn1-Like Protein 1General Control Of Amino-Acid Synthesis 1-Like Protein 1Translational Activator Gcn1Hsgcn1 |
| Database Links | Reactome: R-HSA-9633012 |
| Cellular Localisation | CytoplasmAssociates With Ribosomes In Undifferentiated Neuroblastoma Cells And Increases After Neuronal Differentiation |
| Alternative Antibody Names | Anti-Stalled Ribosome Sensor Gcn1 antibodyAnti-Gcn1 Eif-2-Alpha Kinase Activator Homolog antibodyAnti-Gcn1-Like Protein 1 antibodyAnti-General Control Of Amino-Acid Synthesis 1-Like Protein 1 antibodyAnti-Translational Activator Gcn1 antibodyAnti-Hsgcn1 antibodyAnti-GCN1 antibodyAnti-GCN1L1 antibodyAnti-KIAA0219 antibody |
Information sourced from Uniprot.org