• Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T (KO) cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ115042) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GCN1L1 antibody (2462-2671) (STJ115042)

SKU:
STJ115042

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GCN1L1 (2462-2671) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCN1
Gene ID: 10985
Uniprot ID: GCN1_HUMAN
Immunogen Region: 2462-2671
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2462-2671 of human GCN1L1 (NP_006827.1).
Immunogen Sequence: LSAVLQQCLLADVSGIDWMV RHGRSLALSVAVNVAPGRLC AGRYSSDVQEMILSSATADR IPIAVSGVRGMGFLMRHHIE TGGGQLPAKLSSLFVKCLQN PSSDIRLVAEKMIWWANKDP LPPLDPQAIKPILKALLDNT KDKNTVVRAYSDQAIVNLLK MRQGEEVFQSLSKILDVASL EVLNEVNRRSLKKLASQADS TEQVDDTILT
Tissue Specificity Ubiquitously expressed. Expressed in skeletal muscules, ovary and testis.
Function Ribosome collision sensor that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. Directly binds to the ribosome and acts as a sentinel for colliding ribosomes: activated following ribosome stalling and promotes recruitment of RNF14, which directly ubiquitinates EEF1A1/eEF1A, leading to its degradation. In addition to EEF1A1/eEF1A, the RNF14-RNF25 translation quality control pathway mediates degradation of ETF1/eRF1 and ubiquitination of ribosomal protein. GCN1 also acts as a positive activator of the integrated stress response (ISR) by mediating activation of EIF2AK4/GCN2 in response to amino acid starvation. Interaction with EIF2AK4/GCN2 on translating ribosomes stimulates EIF2AK4/GCN2 kinase activity, leading to phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1). EIF2S1/eIF-2-alpha phosphorylation converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion.
Protein Name Stalled Ribosome Sensor Gcn1
Gcn1 Eif-2-Alpha Kinase Activator Homolog
Gcn1-Like Protein 1
General Control Of Amino-Acid Synthesis 1-Like Protein 1
Translational Activator Gcn1
Hsgcn1
Database Links Reactome: R-HSA-9633012
Cellular Localisation Cytoplasm
Associates With Ribosomes In Undifferentiated Neuroblastoma Cells And Increases After Neuronal Differentiation
Alternative Antibody Names Anti-Stalled Ribosome Sensor Gcn1 antibody
Anti-Gcn1 Eif-2-Alpha Kinase Activator Homolog antibody
Anti-Gcn1-Like Protein 1 antibody
Anti-General Control Of Amino-Acid Synthesis 1-Like Protein 1 antibody
Anti-Translational Activator Gcn1 antibody
Anti-Hsgcn1 antibody
Anti-GCN1 antibody
Anti-GCN1L1 antibody
Anti-KIAA0219 antibody

Information sourced from Uniprot.org

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