• Immunofluorescence analysis of C6 cells using GCAT Rabbit polyclonal antibody (STJ118247) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GCAT in paraffin-embedded human colon carcinoma using GCAT Rabbit polyclonal antibody (STJ118247) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from mouse eye, using GCAT Rabbit polyclonal antibody (STJ118247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GCAT antibody (170-419) (STJ118247)

SKU:
STJ118247

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GCAT (170-419) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCAT
Gene ID: 23464
Uniprot ID: KBL_HUMAN
Immunogen Region: 170-419
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 170-419 of human GCAT (NP_055106.1).
Immunogen Sequence: KAHKYRYRHLDMADLEAKLQ EAQKHRLRLVATDGAFSMDG DIAPLQEICCLASRYGALVF MDECHATGFLGPTGRGTDEL LGVMDQVTIINSTLGKALGG ASGGYTTGPGPLVSLLRQRA RPYLFSNSLPPAVVGCASKA LDLLMGSNTIVQSMAAKTQR FRSKMEAAGFTISGASHPIC PVMLGDARLASRMADDMLKR GIFVIGFSYPVVPKGKARIR VQISAVHSEEDIDRCVEAF
Tissue Specificity Strongly expressed in heart, brain, liver and pancreas. Also found in lung.
Function Pyridoxal phosphate (PLP) dependent enzyme, which catalyzes the cleavage of 2-amino-3-oxobutanoate to glycine and acetyl-CoA.
Protein Name 2-Amino-3-Ketobutyrate Coenzyme A Ligase - Mitochondrial
Akb Ligase
Aminoacetone Synthase
Glycine Acetyltransferase
Database Links Reactome: R-HSA-8849175
Cellular Localisation Mitochondrion
Nucleus
Translocates To The Nucleus Upon Cold And Osmotic Stress
Alternative Antibody Names Anti-2-Amino-3-Ketobutyrate Coenzyme A Ligase - Mitochondrial antibody
Anti-Akb Ligase antibody
Anti-Aminoacetone Synthase antibody
Anti-Glycine Acetyltransferase antibody
Anti-GCAT antibody
Anti-KBL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance