• Western blot analysis of lysates from 293 cells, using GAK Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from HepG2 cells using GAK antibody.
  • Western blot analysis of various cells using GAK Polyclonal Antibody

Anti-GAK antibody (101-150 aa) (STJ93199)

SKU:
STJ93199

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cyclin-G-associated kinase (101-150 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GAK
Gene ID: 2580
Uniprot ID: GAK_HUMAN
Immunogen Region: 101-150 aa
Specificity: GAK Polyclonal Antibody detects endogenous levels of GAK protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GAK at the amino acid range 101-150
Function Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Protein Name Cyclin-G-Associated Kinase
Database Links Reactome: R-HSA-432722
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Perinuclear Region
Golgi Apparatus
Trans-Golgi Network
Cell Junction
Focal Adhesion
Localizes To The Perinuclear Area And To The Trans-Golgi Network
Also Seen On The Plasma Membrane
Probably At Focal Adhesions
Alternative Antibody Names Anti-Cyclin-G-Associated Kinase antibody
Anti-GAK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance