Anti-GABRB3 antibody (Cytoplasmic Loop) (STJA0003647)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenFusion protein from the cytoplasmic loop of the beta 3 subunit.
General Information
| Short Description | Rabbit polyclonal anti-GABAA Receptor b3 (Cytoplasmic Loop) for use in WB and IHC in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000IHC 1:300 |
| Formulation | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Gabrb3 |
| Gene ID | 24922 |
| Uniprot ID | GBRB3_RAT |
| Immunogen | Fusion protein from the cytoplasmic loop of the beta 3 subunit. |
| Immunogen Region | Cytoplasmic Loop |
Additional Info
| Tissue Specificity | Expressed in brain (at protein level), in cerebellar granule cells. |
| Function | Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). GABAARs containing beta-3/GABRB3 subunit are found at both synaptic and extrasynaptic sites. When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. GABAARs containing alpha-1 and beta-3 subunits exhibit synaptogenic activity.the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation. Extrasynaptic beta-3 receptors contribute to the tonic GABAergic inhibition. GABAARs containing alpha-1, beta-3 and epsilon subunits may permit spontaneous chloride channel activity while preserving the structural information required for GABA-gated openings. Beta-containing GABAARs can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness. Plays an important role in somatosensation and in the production of antinociception. |
| Protein Name | Gamma-Aminobutyric Acid Receptor Subunit Beta-3Gaba(A Receptor Subunit Beta-3Gabaar Subunit Beta-3 |
| Database Links | Reactome: R-RNO-977443 |
| Cellular Localisation | Postsynaptic Cell MembraneMulti-Pass Membrane ProteinCell MembraneCytoplasmic Vesicle MembraneThe Beta-3 Subunits Are Present In Golgi Synapses And On The Extrasynaptic Membranes (When Coassembled With Alpha-1/6 And Gamma-2 Subunits)And In Some Of The Mossy Fiber To Granule Cell Synapses (When Coassembled With Alpha-6 And Gamma-2 Subunits)The Beta-3 SubunitsWhen Coassembled With Alpha-6 And Delta SubunitsAre Found Only On The Extrasynaptic Somatic And Dendritic Membranes |
| Alternative Antibody Names | Anti-Gamma-Aminobutyric Acid Receptor Subunit Beta-3 antibodyAnti-Gaba(A Receptor Subunit Beta-3 antibodyAnti-Gabaar Subunit Beta-3 antibodyAnti-Gabrb3 antibodyAnti-Gabrb-3 antibody |
Information sourced from Uniprot.org