| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | 1-349 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of zebrafish FTO (XP_009301626.1). |
| Immunogen Sequence: | MKPRQRKQYFRNMKRSDDSE REKRRKRRRLLQELGEQRIP YLSPTDPGFQDLWDSSYAGL ALRQSGTLPEGLHEKVQSAL LTLQRHGCLLRDLVRVRDRD VFTAVSRALVGQPGYTYRYL DTRLFTIPWHCEGEEGQKDE KGKPCCDSDLRDACKALWEL NQFFCSDVKQQTNARGVKRT RSDTENSEDAPGEGMCEEES VKDRLVEEKTIEEEEEDSGQ GCSHSSPPSSTPPAAQASL |
| Background | Predicted to enable 2-oxoglutarate-dependent dioxygenase activity and ferrous iron binding activity. Acts upstream of or within several processes, including cilium assembly; left/right pattern formation; and positive regulation of canonical Wnt signaling pathway. Predicted to be located in cytoplasm and nuclear speck. Human ortholog (s) of this gene implicated in obesity. Orthologous to human FTO (FTO alpha-ketoglutarate dependent dioxygenase). |
Information sourced from Uniprot.org