• Immunofluorescence analysis of A-549 cells using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded rat kidney tissue using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded mouse kidney tissue using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded human liver tissue using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FTH1 antibody (1-183) (STJ23718)

SKU:
STJ23718

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ferritin Heavy Chain (1-183) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FTH1
Gene ID: 2495
Uniprot ID: FRIH_HUMAN
Immunogen Region: 1-183
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human Ferritin Heavy Chain (NP_002023.2).
Immunogen Sequence: MTTASTSQVRQNYHQDSEAA INRQINLELYASYVYLSMSY YFDRDDVALKNFAKYFLHQS HEEREHAEKLMKLQNQRGGR IFLQDIKKPDCDDWESGLNA MECALHLEKNVNQSLLELHK LATDKNDPHLCDFIETHYLN EQVKAIKELGDHVTNLRKMG APESGLAEYLFDKHTLGDSD NES
Tissue Specificity Expressed in the liver.
Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Protein Name Ferritin Heavy Chain
Ferritin H Subunit
Cell Proliferation-Inducing Gene 15 Protein Cleaved Into - Ferritin Heavy Chain - N-Terminally Processed
Database Links Reactome: R-HSA-3000480
Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-917937
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ferritin Heavy Chain antibody
Anti-Ferritin H Subunit antibody
Anti-Cell Proliferation-Inducing Gene 15 Protein Cleaved Into - Ferritin Heavy Chain - N-Terminally Processed antibody
Anti-FTH1 antibody
Anti-FTH antibody
Anti-FTHL6 antibody
Anti-OK antibody
Anti-SW-cl.84 antibody
Anti-PIG15 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance