• Immunofluorescence analysis of A-549 cells using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded rat kidney tissue using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded mouse kidney tissue using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ferritin Heavy Chain in paraffin-embedded human liver tissue using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Ferritin Heavy Chain Rabbit polyclonal antibody (STJ23718) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FTH1 antibody (1-183) (STJ23718)

SKU:
STJ23718

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FTH1
Gene ID: 2495
Uniprot ID: FRIH_HUMAN
Immunogen Region: 1-183
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human Ferritin Heavy Chain (NP_002023.2).
Immunogen Sequence: MTTASTSQVRQNYHQDSEAA INRQINLELYASYVYLSMSY YFDRDDVALKNFAKYFLHQS HEEREHAEKLMKLQNQRGGR IFLQDIKKPDCDDWESGLNA MECALHLEKNVNQSLLELHK LATDKNDPHLCDFIETHYLN EQVKAIKELGDHVTNLRKMG APESGLAEYLFDKHTLGDSD NES
Tissue Specificity Expressed in the liver.
Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron.
Protein Name Ferritin Heavy Chain
Ferritin H Subunit
Cell Proliferation-Inducing Gene 15 Protein Cleaved Into - Ferritin Heavy Chain - N-Terminally Processed
Database Links Reactome: R-HSA-3000480
Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-917937
Cellular Localisation Cytoplasm
Lysosome
Cytoplasmic Vesicle
Autophagosome
Alternative Antibody Names Anti-Ferritin Heavy Chain antibody
Anti-Ferritin H Subunit antibody
Anti-Cell Proliferation-Inducing Gene 15 Protein Cleaved Into - Ferritin Heavy Chain - N-Terminally Processed antibody
Anti-FTH1 antibody
Anti-FTH antibody
Anti-FTHL6 antibody
Anti-OK antibody
Anti-SW-cl.84 antibody
Anti-PIG15 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance