• Western blot analysis of extracts of various cell lines, using FRMD6 antibody (STJ112036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U2OS cells using FRMD6 antibody (STJ112036) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FRMD6 antibody (STJ112036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of C6 cells using FRMD6 antibody (STJ112036) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded Rat heart using FRMD6 antibody (STJ112036) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded Mouse heart using FRMD6 antibody (STJ112036) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human thyroid cancer using FRMD6 antibody (STJ112036) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using FRMD6 antibody (STJ112036) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse heart using FRMD6 antibody (STJ112036) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded Rat heart using FRMD6 antibody (STJ112036) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using FRMD6 antibody (STJ112036) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FRMD6 antibody (STJ112036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U2OS cells using FRMD6 antibody (STJ112036) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FRMD6 antibody (STJ112036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FRMD6 antibody (82-183) (STJ112036)

SKU:
STJ112036

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FRMD6 (82-183) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FRMD6
Gene ID: 122786
Uniprot ID: FRMD6_HUMAN
Immunogen Region: 82-183
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 82-183 of human FRMD6 (NP_001253975.1).
Immunogen Sequence: KEWKKEASKVRQYEVTWGID QFGPPMIIHFRVQYYVENGR LISDRAARYYYYWHLRKQVL HSQCVLREEAYFLLAAFALQ ADLGNFKRNKHYGKYFEPEA YF
Protein Name Ferm Domain-Containing Protein 6
Willin
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Can Colocalize With Actin
Alternative Antibody Names Anti-Ferm Domain-Containing Protein 6 antibody
Anti-Willin antibody
Anti-FRMD6 antibody
Anti-C14orf31 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance