• STJ70110 (1µg/ml) staining of Human Muscle (A) , (2ug/ml) MOLT4 (B) and (1µg/ml) negative control Pancreas (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70110 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70110 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ70110 staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).
  • STJ70110 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells. This data is from a previous batch, not on sale.

Anti-FOXP3/SCURFIN antibody (C-Term) (STJ70110)

SKU:
STJ70110-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/ICC/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-FOXP3/SCURFIN (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunocytochemistry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IF-Strong expression of the protein seen in the nucleoplasm of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: FOXP3
Gene ID: 50943
Uniprot ID: FOXP3_HUMAN
Immunogen Region: C-Term
Accession Number: NP_054728.2; NP_001107849.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
Immunogen Sequence: SQRPSRCSNPTPGP
Function Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7. Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1. Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development. Inhibits the transcriptional activator activity of RORA. Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.
Protein Name Forkhead Box Protein P3
Scurfin Cleaved Into - Forkhead Box Protein P3 - C-Terminally Processed - Forkhead Box Protein P3 41 Kda Form
Database Links Reactome: R-HSA-8877330
Reactome: R-HSA-8939256
Cellular Localisation Nucleus
Cytoplasm
Predominantly Expressed In The Cytoplasm In Activated Conventional T-Cells Whereas Predominantly Expressed In The Nucleus In Regulatory T-Cells (Treg)
The 41 Kda Form Derived By Proteolytic Processing Is Found Exclusively In The Chromatin Fraction Of Activated Treg Cells
Alternative Antibody Names Anti-Forkhead Box Protein P3 antibody
Anti-Scurfin Cleaved Into - Forkhead Box Protein P3 - C-Terminally Processed - Forkhead Box Protein P3 41 Kda Form antibody
Anti-FOXP3 antibody
Anti-IPEX antibody
Anti-JM2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance