• Biotinylated STJ70184 (1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Flotillin 1, Biotinylated antibody (C-Term) {Biotin} (STJ73282)

SKU:
STJ73282-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Flotillin 1, Biotinylated (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:2000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: FLOT1
Gene ID: 10211
Uniprot ID: FLOT1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_005794.1; NP_001305804.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_005794.1; NP_001305804.1).
Immunogen Sequence: CSISQVNHKPLRTA.
Protein Name Flotillin-1
Database Links Reactome: R-HSA-5213460
Reactome: R-HSA-5675482
Reactome: R-HSA-8849932
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Endosome
Membrane
Caveola
Melanosome
Membrane Raft
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Membrane-Associated Protein Of Caveola
Alternative Antibody Names Anti-Flotillin-1 antibody
Anti-FLOT1 antibody

Information sourced from Uniprot.org

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