| Short Description : | Mouse monoclonal anti-Flag-Tag for use in WB and IP in Species-independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IP |
| Host: | Mouse |
| Reactivity: | Species-independent |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 10E1-1D8-5F5 |
| Isotype: | IgG2b |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Affinity Purified |
| Dilution Range: | WB 1:1000-1:10000IP 1:500 |
| Formulation: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Immunogen: | Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK) , coupled to KLH. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. |
Information sourced from Uniprot.org
