Anti-Flag-Tag antibody [10E1-1D8-5F5] (STJA0032989)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenMonoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK) , coupled to KLH.
General Information
| Short Description | Mouse monoclonal anti-Flag-Tag for use in WB and IP in Species-independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Mouse |
| Reactivity | Species-independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 10E1-1D8-5F5 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:1000-1:10000IP 1:500 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Immunogen | Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK) , coupled to KLH. |
Additional Info
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. |
Information sourced from Uniprot.org