Anti-FHL1/SLIM1 antibody (Internal) (STJ70598)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70598-100
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General Information

Short DescriptionGoat polyclonal antibody anti-FHL1/SLIM1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/WB/IF/FC
HostGoat
ReactivityHuman/Mouse/Rat/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 32kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates and in Rat Lung and Testis lysates (calculated MW of 31.9kDa according to Mouse NP_001274729.1 and Rat NP_001
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolFHL1
Gene ID2273
Uniprot IDFHL1_HUMAN
Accession NumberNP_001440.2; NP_001153171.1
Immunogen RegionInternal
Immunogen SequenceCNKRFVFHNEQVY
SpecificityThis antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variant

Additional Info

Function May have an involvement in muscle development or hypertrophy.
Protein Name Four And A Half Lim Domains Protein 1
Fhl-1
Skeletal Muscle Lim-Protein 1
Slim
Slim-1
Cellular Localisation Isoform 1: Cytoplasm
Isoform 3: Cytoplasm
Nucleus
Isoform 2: Nucleus
Cytoplasm
Cytosol
Predominantly Nuclear In Myoblasts But Is Cytosolic In Differentiated Myotubes
Alternative Antibody Names Anti-Four And A Half Lim Domains Protein 1 antibody
Anti-Fhl-1 antibody
Anti-Skeletal Muscle Lim-Protein 1 antibody
Anti-Slim antibody
Anti-Slim-1 antibody
Anti-FHL1 antibody
Anti-SLIM1 antibody

Information sourced from Uniprot.org

Citations

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