• Immunofluorescence analysis of NIH-3T3 cells using SQS/FDFT1 antibody (STJ27985) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using SQS/FDFT1 antibody (STJ27985) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using SQS/FDFT1 antibody (STJ27985) at dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SQS/FDFT1 antibody (STJ27985) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.

Anti-FDFT1 antibody (1-260) (STJ27985)

SKU:
STJ27985

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SQS/FDFT1 (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FDFT1
Gene ID: 2222
Uniprot ID: FDFT_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SQS/FDFT1 (NP_004453.3).
Immunogen Sequence: MEFVKCLGHPEEFYNLVRFR IGGKRKVMPKMDQDSLSSSL KTCYKYLNQTSRSFAAVIQA LDGEMRNAVCIFYLVLRALD TLEDDMTISVEKKVPLLHNF HSFLYQPDWRFMESKEKDRQ VLEDFPTISLEFRNLAEKYQ TVIADICRRMGIGMAEFLDK HVTSEQEWDKYCHYVAGLVG IGLSRLFSASEFEDPLVGED TERANSMGLFLQKTNIIRDY LEDQQGGREFWPQEVWSRY
Tissue Specificity Widely expressed.
Function Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
Protein Name Squalene Synthase
Sqs
Ss
Fpp -Fpp Farnesyltransferase
Farnesyl-Diphosphate Farnesyltransferase
Farnesyl-Diphosphate Farnesyltransferase 1
Database Links Reactome: R-HSA-191273
Reactome: R-HSA-1989781
Reactome: R-HSA-2426168
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Squalene Synthase antibody
Anti-Sqs antibody
Anti-Ss antibody
Anti-Fpp -Fpp Farnesyltransferase antibody
Anti-Farnesyl-Diphosphate Farnesyltransferase antibody
Anti-Farnesyl-Diphosphate Farnesyltransferase 1 antibody
Anti-FDFT1 antibody

Information sourced from Uniprot.org

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