Anti-FBXW5 antibody (184-566) (STJ111668)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FBXW5 (184-566) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FBXW5 |
| Gene ID | 54461 |
| Uniprot ID | FBXW5_HUMAN |
| Immunogen Region | 184-566 |
| Immunogen Sequence | VRNKPYDVFGCWLTETSLIS GNLHRIGDITSCSVLWLNNA FQDVESENVNVVKRLFKIQN LNASTVRTVMVADCSRFDSP DLLLEAGDPATSPCRIFDLG SDNEEVVAGPAPAHAKEGLR HFLDRVLEGRAQPQLSERML ETKVAELLAQGHTKPPERSA TGAKSKYLIFTTGCLTYSPH QIGIKQILPHQMTTAGPVLG EGRGSDAFFDALDHVIDIHG HIIGMGLSPDNRYLYVNSR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 184-566 of human FBXW5 (NP_061871.1). |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-151 by PLK4 during the G1/S transition, leading to inhibit its ability to ubiquitinate SASS6. Ubiquitinated and degraded by the APC/C complex during mitosis and G1 phase. |
| Function | Substrate recognition component of both SCF (SKP1-CUL1-F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. The SCF(FBXW5) complex also mediates ubiquitination and degradation of actin-regulator EPS8 during G2 phase, leading to the transient degradation of EPS8 and subsequent cell shape changes required to allow mitotic progression. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway. |
| Protein Name | F-Box/Wd Repeat-Containing Protein 5F-Box And Wd-40 Domain-Containing Protein 5 |
| Database Links | Reactome: R-HSA-390471Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-F-Box/Wd Repeat-Containing Protein 5 antibodyAnti-F-Box And Wd-40 Domain-Containing Protein 5 antibodyAnti-FBXW5 antibodyAnti-FBW5 antibodyAnti-PP3971 antibody |
Information sourced from Uniprot.org