| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FBXW5 (184-566) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FBXW5 |
| Gene ID: |
54461 |
| Uniprot ID: |
FBXW5_HUMAN |
| Immunogen Region: |
184-566 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 184-566 of human FBXW5 (NP_061871.1). |
| Immunogen Sequence: |
VRNKPYDVFGCWLTETSLIS GNLHRIGDITSCSVLWLNNA FQDVESENVNVVKRLFKIQN LNASTVRTVMVADCSRFDSP DLLLEAGDPATSPCRIFDLG SDNEEVVAGPAPAHAKEGLR HFLDRVLEGRAQPQLSERML ETKVAELLAQGHTKPPERSA TGAKSKYLIFTTGCLTYSPH QIGIKQILPHQMTTAGPVLG EGRGSDAFFDALDHVIDIHG HIIGMGLSPDNRYLYVNSR |
| Post Translational Modifications | Phosphorylated at Ser-151 by PLK4 during the G1/S transition, leading to inhibit its ability to ubiquitinate SASS6. Ubiquitinated and degraded by the APC/C complex during mitosis and G1 phase. |
| Function | Substrate recognition component of both SCF (SKP1-CUL1-F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. The SCF(FBXW5) complex also mediates ubiquitination and degradation of actin-regulator EPS8 during G2 phase, leading to the transient degradation of EPS8 and subsequent cell shape changes required to allow mitotic progression. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway. |
| Protein Name | F-Box/Wd Repeat-Containing Protein 5F-Box And Wd-40 Domain-Containing Protein 5 |
| Database Links | Reactome: R-HSA-390471Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-F-Box/Wd Repeat-Containing Protein 5 antibodyAnti-F-Box And Wd-40 Domain-Containing Protein 5 antibodyAnti-FBXW5 antibodyAnti-FBW5 antibodyAnti-PP3971 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
