• Immunofluorescence analysis of L929 cells using FBXO21 Rabbit polyclonal antibody (STJ118547) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of FBXO21 in paraffin-embedded Human liver cancer using FBXO21 Rabbit polyclonal antibody (STJ118547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FBXO21 in paraffin-embedded Rat lung using FBXO21 Rabbit polyclonal antibody (STJ118547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FBXO21 in paraffin-embedded Mouse kidney using FBXO21 Rabbit polyclonal antibody (STJ118547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FBXO21 Rabbit polyclonal antibody (STJ118547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FBXO21 antibody (440-621) (STJ118547)

SKU:
STJ118547

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FBXO21 (440-621) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBXO21
Gene ID: 23014
Uniprot ID: FBX21_HUMAN
Immunogen Region: 440-621
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 440-621 of human FBXO21 (NP_055817.1).
Immunogen Sequence: PEKVLDILQHIQTLDPGQHG AVGYLVQHTLEHIERKKEEV GVEVKLRSDEKHRDVCYSIG LIMKHKRYGYNCVIYGWDPT CMMGHEWIRNMNVHSLPHGH HQPFYNVLVEDGSCRYAAQE NLEYNVEPQEISHPDVGRYF SEFTGTHYIPNAELEIRYPE DLEFVYETVQNIYSAKKENI DE
Function Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Protein Name F-Box Only Protein 21
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Alternative Antibody Names Anti-F-Box Only Protein 21 antibody
Anti-FBXO21 antibody
Anti-FBX21 antibody
Anti-KIAA0875 antibody

Information sourced from Uniprot.org

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