• Western blot analysis of extracts of various cell lines, using FBP2 antibody (STJ111892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of Rat skeletal muscle using FBP2 antibody (STJ111892) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse skeletal muscle using FBP2 antibody (STJ111892) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Mouse skeletal muscle using FBP2 antibody (STJ111892) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat skeletal muscle using FBP2 antibody (STJ111892) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FBP2 antibody (STJ111892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FBP2 antibody (1-339) (STJ111892)

SKU:
STJ111892

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FBP2 (1-339) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBP2
Gene ID: 8789
Uniprot ID: F16P2_HUMAN
Immunogen Region: 1-339
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human FBP2 (NP_003828.2).
Immunogen Sequence: MTDRSPFETDMLTLTRYVME KGRQAKGTGELTQLLNSMLT AIKAISSAVRKAGLAHLYGI AGSVNVTGDEVKKLDVLSNS LVINMVQSSYSTCVLVSEEN KDAIITAKEKRGKYVVCFDP LDGSSNIDCLASIGTIFAIY RKTSEDEPSEKDALQCGRNI VAAGYALYGSATLVALSTGQ GVDLFMLDPALGEFVLVEKD VKIKKKGKIYSLNEGYAKYF DAATTEYVQKKKFPEDGSA
Tissue Specificity Expressed in skeletal muscle (at protein level).
Function Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.
Protein Name Fructose-1 -6-Bisphosphatase Isozyme 2
Fbpase 2
D-Fructose-1 -6-Bisphosphate 1-Phosphohydrolase 2
Muscle Fbpase
Database Links Reactome: R-HSA-70263
Cellular Localisation Cell Junction
Cytoplasm
Nucleus
Myofibril
Sarcomere
Z Line
In Neonatal Cardiomyocytes
Distributed Throughout The Cytosol
Accumulating In The Intercalated Disks Which Occur At The Z Line Of Cardiomyocytes And Connect Adjacent Cells
And Also Located In The Nucleus
Dissociates From The Z Line Following An Increase In Cytosolic Ca(2+) Concentration
In Muscle Precursor Cells
Localizes Predominantly To The Nucleus And To A Lesser Extent To The Cytoplasm At The Proliferative Phase
While Mainly Localizing To The Cytoplasm At The Differentiation Phase
Colocalizes With Aldoa And Alpha-Actinin On Both Sides Of The Z Line Of Skeletal Muscle
Dissociates Rapidly From The Z Line Following An Increase In Cytosolic Ca(2+) Concentration
Alternative Antibody Names Anti-Fructose-1 -6-Bisphosphatase Isozyme 2 antibody
Anti-Fbpase 2 antibody
Anti-D-Fructose-1 -6-Bisphosphate 1-Phosphohydrolase 2 antibody
Anti-Muscle Fbpase antibody
Anti-FBP2 antibody

Information sourced from Uniprot.org

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