Anti-FAM38A/PIEZO1 antibody (1811-1952 aa) (STJ11107060)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FAM38A/PIEZO1 (1811-1952 aa) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIEZO1 |
| Gene ID | 9780 |
| Uniprot ID | PIEZ1_HUMAN |
| Immunogen Region | 1811-1952 aa |
| Immunogen Sequence | DSPSKEHDKSGEEEQGAEEG PGVPAATTEDHIQVEARVGP TDGTPEPQVELRPRDTRRIS LRFRRRKKEGPARKGAAAIE AEDREEEEGEEEKEAPTGRE KRPSRSGGRVRAAGRRLQGF CLSLAQGTYRPLRRFFHDIL HT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1811-1952 of human FAM38A/PIEZO1 (NP_001136336.2). |
Additional Info
| Tissue Specificity | Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level). Expressed in myoblasts (at protein level). |
| Function | Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain. Piezo channels are homotrimeric three-blade propeller-shaped structures that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Conductance to monovalent alkali ions is highest for K(+), intermediate for Na(+) and lowest for Li(+). Divalent ions except for Mn(2+) permeate the channel but more slowly than the monovalent ions and they also reduce K(+) currents. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In inner ear hair cells, PIEZO1/2 subunits may constitute part of the mechanotransducer (MET) non-selective cation channel complex where they may act as pore-forming ion-conducting component in the complex. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as a shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology. Acts as a sensor of phosphatidylserine (PS) flipping at the plasma membrane and governs morphogenesis of muscle cells. In myoblasts, flippase-mediated PS enrichment at the inner leaflet of plasma membrane triggers channel activation and Ca2+ influx followed by Rho GTPases signal transduction, leading to assembly of cortical actomyosin fibers and myotube formation. |
| Protein Name | Piezo-Type Mechanosensitive Ion Channel Component 1Membrane Protein Induced By Beta-Amyloid TreatmentMibProtein Fam38a |
| Database Links | Reactome: R-HSA-9856530Reactome: R-HSA-9856532Reactome: R-HSA-9860927 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum-Golgi Intermediate Compartment MembraneCell MembraneCell ProjectionLamellipodium MembraneIn ErythrocytesLocated In The Plasma MembraneAccumulates At The Leading Apical Lamellipodia Of Endothelial Cells In Response To Shear StressColocalizes With F-Actin And Myh9 At The Actomyosin Cortex In Myoblasts |
| Alternative Antibody Names | Anti-Piezo-Type Mechanosensitive Ion Channel Component 1 antibodyAnti-Membrane Protein Induced By Beta-Amyloid Treatment antibodyAnti-Mib antibodyAnti-Protein Fam38a antibodyAnti-PIEZO1 antibodyAnti-FAM38A antibodyAnti-KIAA0233 antibody |
Information sourced from Uniprot.org