Anti-FADS1 antibody (101-114 aa) [R02-1F0] (STJA0012373)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human FADS1
General Information
| Short Description | Rabbit monoclonal anti-FADS1 (101-114 aa) for use in WB, IHC-F, IHC-P, ICC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-1F0 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | FADS1 |
| Gene ID | 3992 |
| Uniprot ID | FADS1_HUMAN |
| Immunogen | A synthetic peptide of human FADS1 |
| Immunogen Region | 101-114 aa |
Additional Info
| Tissue Specificity | Widely expressed, with highest levels in liver, brain, adrenal gland and heart. Highly expressed in fetal liver and brain. |
| Function | Isoform 1: Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Specifically, desaturates dihomo-gamma-linoleoate (DGLA) (20:3n-6) and eicosatetraenoate (ETA) (20:4n-3) to generate arachidonate (AA) (20:4n-6) and eicosapentaenoate (EPA) (20:5n-3), respectively. As a rate limiting enzyme for DGLA (20:3n-6) and AA (20:4n-6)-derived eicosanoid biosynthesis, controls the metabolism of inflammatory lipids like prostaglandin E2, critical for efficient acute inflammatory response and maintenance of epithelium homeostasis. Contributes to membrane phospholipid biosynthesis by providing AA (20:4n-6) as a major acyl chain esterified into phospholipids. In particular, regulates phosphatidylinositol-4,5-bisphosphate levels, modulating inflammatory cytokine production in T-cells. Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6). Isoform 2: Does not exhibit any catalytic activity toward 20:3n-6, but it may enhance FADS2 activity. |
| Protein Name | Acyl-Coa8-3-DesaturaseDelta(5 Fatty Acid DesaturaseD5dDelta(5 DesaturaseDelta-5 DesaturaseFatty Acid Desaturase 1 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2046105Reactome: R-HSA-2046106 |
| Cellular Localisation | Isoform 1: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinMitochondrionIsoform 2: Endoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-Acyl-Coa antibodyAnti-8-3-Desaturase antibodyAnti-Delta(5 Fatty Acid Desaturase antibodyAnti-D5d antibodyAnti-Delta(5 Desaturase antibodyAnti-Delta-5 Desaturase antibodyAnti-Fatty Acid Desaturase 1 antibodyAnti-FADS1 antibodyAnti-FADSD5 antibody |
Information sourced from Uniprot.org