| Short Description : | Mouse monoclonal anti-ETS translocation variant 1 (1-191 aa) for use in WB and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 1C8B6 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000ELISA 1:10000 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ETV1 |
| Gene ID: | 2115 |
| Uniprot ID: | ETV1_HUMAN |
| Immunogen: | Purified recombinant fragment of ER81 (aa1-191) expressed in E. Coli. |
| Immunogen Region: | 1-191 aa |
| Specificity: | ER81 Monoclonal Antibody detects endogenous levels of ER81 protein. |
| Post Translational Modifications | Sumoylated. Phosphorylated at Ser-191 and Ser-216 by RPS6KA1 and RPS6KA5.phosphorylation activates transcriptional activity. |
| Function | Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGAAT-3'. Required for olfactory dopaminergic neuron differentiation.may directly activate expression of tyrosine hydroxylase (TH). |
| Protein Name | Ets Translocation Variant 1Ets-Related Protein 81 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets Translocation Variant 1 antibodyAnti-Ets-Related Protein 81 antibodyAnti-ETV1 antibodyAnti-ER81 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186370/408075/STJ11107174_1__85519.1744894498.jpg?c=1 "Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.")
