• Immunohistochemistry analysis of ESPN in paraffin-embedded Human colon carcinoma using ESPN Rabbit polyclonal antibody (STJ118353) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ESPN Rabbit polyclonal antibody (STJ118353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ESPN antibody (585-854) (STJ118353)

SKU:
STJ118353

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ESPN (585-854) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ESPN
Gene ID: 83715
Uniprot ID: ESPN_HUMAN
Immunogen Region: 585-854
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 585-854 of human ESPN (NP_113663.2).
Immunogen Sequence: CAADPKASRELPPPPPPPPP PLPEAASSPPPAPPLPLESA GPGCGQRRSSSSTGSTKSFN MMSPTGDNSELLAEIKAGKS LKPTPQSKGLTTVFSGIGQP AFQPDSPLPSVSPALSPVRS PTPPAAGFQPLLNGSLVPVP PTTPAPGVQLDVEALIPTHD EQGRPIPEWKRQVMVRKMQL KMQEEEEQRRKEEEEEARLA SMPAWRRDLLRKKLEEEREQ KRKEEERQKQEELRREKEQ
Function Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimension, dynamics and signaling capacities of the actin filament-rich microvilli in the mechanosensory and chemosensory cells. Required for the assembly and stabilization of the stereociliary parallel actin bundles. Plays a crucial role in the formation and maintenance of inner ear hair cell stereocilia. Involved in the elongation of actin in stereocilia. In extrastriolar hair cells, required for targeting MYO3B to stereocilia tips, and for regulation of stereocilia diameter and staircase formation.
Protein Name Espin
Autosomal Recessive Deafness Type 36 Protein
Ectoplasmic Specialization Protein
Database Links Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Stereocilium
Microvillus
Alternative Antibody Names Anti-Espin antibody
Anti-Autosomal Recessive Deafness Type 36 Protein antibody
Anti-Ectoplasmic Specialization Protein antibody
Anti-ESPN antibody
Anti-DFNB36 antibody
Anti-LP2654 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance