• Western blot analysis of extracts of various cell lines, using ERLIN1 antibody (STJ117043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using ERLIN1 antibody (STJ117043) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300 Mu g extracts of DU145 cells using 3 Mu g ERLIN1 antibody (STJ117043). Western blot was performed from the immunoprecipitate using ERLIN1 antibody (STJ117043) at a dilution of 1:1000.

Anti-ERLIN1 antibody (259-348) (STJ117043)

SKU:
STJ117043

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ERLIN1 (259-348) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERLIN1
Gene ID: 10613
Uniprot ID: ERLN1_HUMAN
Immunogen Region: 259-348
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2).
Immunogen Sequence: KADAEYYAAHKYATSNKHKL TPEYLELKKYQAIASNSKIY FGSNIPNMFVDSSCALKYSD IRTGRESSLPSKEALEPSGE NVIQNKESTG
Tissue Specificity Expressed in heart, placenta, liver, kidney, pancreas, prostate, testis, ovary and small intestine.
Function Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. Binds cholesterol and may promote ER retention of the SCAP-SREBF complex. (Microbial infection) Required early in hepatitis C virus (HCV) infection to initiate RNA replication, and later in the infection to support infectious virus production.
Protein Name Erlin-1
Endoplasmic Reticulum Lipid Raft-Associated Protein 1
Protein Ke04
Stomatin-Prohibitin-Flotillin-Hflc/K Domain-Containing Protein 1
Spfh Domain-Containing Protein 1
Database Links Reactome: R-HSA-382556
Reactome: R-HSA-5678895
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Associated With Lipid Raft-Like Domains Of The Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Erlin-1 antibody
Anti-Endoplasmic Reticulum Lipid Raft-Associated Protein 1 antibody
Anti-Protein Ke04 antibody
Anti-Stomatin-Prohibitin-Flotillin-Hflc/K Domain-Containing Protein 1 antibody
Anti-Spfh Domain-Containing Protein 1 antibody
Anti-ERLIN1 antibody
Anti-C10orf69 antibody
Anti-KE04 antibody
Anti-KEO4 antibody
Anti-SPFH1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance