• Western blot analysis of extracts of various cell lines, using ERLIN1 antibody (STJ117043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using ERLIN1 antibody (STJ117043) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300 Mu g extracts of DU145 cells using 3 Mu g ERLIN1 antibody (STJ117043). Western blot was performed from the immunoprecipitate using ERLIN1 antibody (STJ117043) at a dilution of 1:1000.

Anti-ERLIN1 antibody (259-348) (STJ117043)

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STJ117043

$151.88 - $708.08
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Host: Rabbit
Applications: WB/IHC-P/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERLIN1
Gene ID: 10613
Uniprot ID: ERLN1_HUMAN
Immunogen Region: 259-348
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2).
Immunogen Sequence: KADAEYYAAHKYATSNKHKL TPEYLELKKYQAIASNSKIY FGSNIPNMFVDSSCALKYSD IRTGRESSLPSKEALEPSGE NVIQNKESTG
Tissue Specificity Expressed in heart, placenta, liver, kidney, pancreas, prostate, testis, ovary and small intestine.
Post Translational Modifications Deubiquitinated by USP25.leading to stabilization.
Function Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. Binds cholesterol and may promote ER retention of the SCAP-SREBF complex. (Microbial infection) Required early in hepatitis C virus (HCV) infection to initiate RNA replication, and later in the infection to support infectious virus production.
Protein Name Erlin-1
Endoplasmic Reticulum Lipid Raft-Associated Protein 1
Protein Ke04
Stomatin-Prohibitin-Flotillin-Hflc/K Domain-Containing Protein 1
Spfh Domain-Containing Protein 1
Database Links Reactome: R-HSA-382556
Reactome: R-HSA-5678895
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Associated With Lipid Raft-Like Domains Of The Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Erlin-1 antibody
Anti-Endoplasmic Reticulum Lipid Raft-Associated Protein 1 antibody
Anti-Protein Ke04 antibody
Anti-Stomatin-Prohibitin-Flotillin-Hflc/K Domain-Containing Protein 1 antibody
Anti-Spfh Domain-Containing Protein 1 antibody
Anti-ERLIN1 antibody
Anti-C10orf69 antibody
Anti-KE04 antibody
Anti-KEO4 antibody
Anti-SPFH1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance