• HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with STJ72939 (0. 5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
  • STJ72939 (1. 5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).

Anti-ERK3/MAPK6 antibody (Internal) (STJ72939)

SKU:
STJ72939-100

$421.88
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Host: Goat
Applications: Pep-ELISA/WB-Trf/IHC/IP
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ERK3/MAPK6 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: In transfected HEK293 transiently expressing Mouse ERK3/Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands a
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MAPK6
Gene ID: 5597
Uniprot ID: MK06_HUMAN
Immunogen Region: Internal
Accession Number: NP_002739.1
Specificity: Reported variants represent identical protein (NP_056621.4; NP_081694.1).
Immunogen Sequence: HSPVGSPLKSIQ
Post Translational Modifications Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Ubiquitination at Met-1 leads to degradation by the proteasome pathway.
Function Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle.
Protein Name Mitogen-Activated Protein Kinase 6
Map Kinase 6
Mapk 6
Extracellular Signal-Regulated Kinase 3
Erk-3
Map Kinase Isoform P97
P97-Mapk
Database Links Reactome: R-HSA-5687128
Cellular Localisation Cytoplasm
Nucleus
Translocates To The Cytoplasm Following Interaction With Mapkapk5
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 6 antibody
Anti-Map Kinase 6 antibody
Anti-Mapk 6 antibody
Anti-Extracellular Signal-Regulated Kinase 3 antibody
Anti-Erk-3 antibody
Anti-Map Kinase Isoform P97 antibody
Anti-P97-Mapk antibody
Anti-MAPK6 antibody
Anti-ERK3 antibody
Anti-PRKM6 antibody

Information sourced from Uniprot.org

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