| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EPRS (900-1000) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EPRS1 |
| Gene ID: |
2058 |
| Uniprot ID: |
SYEP_HUMAN |
| Immunogen Region: |
900-1000 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human EPRS (NP_004437.2). |
| Immunogen Sequence: |
EAKVLFDKVASQGEVVRKLK TEKAPKDQVDIAVQELLQLK AQYKSLIGVEYKPVSATGAE DKDKKKKEKENKSEKQNKPQ KQNDGQRKDPSKNQGGGLSS S |
| Post Translational Modifications | Phosphorylated at Ser-886 by CDK5. Phosphorylated at Ser-999 by RPS6KB1.triggers EPRS1 release from the aminoacyl-tRNA synthetase multienzyme complex. In monocytes, the IFN-gamma-induced sequential phosphorylation at Ser-886 and Ser-999 releases EPRS1 from the aminoacyl-tRNA synthetase multienzyme complex, allowing its association with the GAIT complex. Phosphorylation at Ser-999 is specifically required for the RPL13A-mediated interaction of the GAIT complex with eIF4G. Phosphorylation at Ser-999 by RPS6KB1, is also induced by insulin through activation of the mTORC1 signaling pathway and promotes the interaction of EPRS1 with SLC27A1. |
| Function | Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. The phosphorylation of EPRS1, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS1 function from protein synthesis to translation inhibition. Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan. |
| Protein Name | Bifunctional Glutamate/Proline--Trna LigaseBifunctional Aminoacyl-Trna SynthetaseCell Proliferation-Inducing Gene 32 ProteinGlutamatyl-Prolyl-Trna Synthetase Includes - Glutamate--Trna LigaseGlutamyl-Trna SynthetaseGlurs - Proline--Trna LigaseProlyl-Trna Synthetase |
| Database Links | Reactome: R-HSA-2408522Reactome: R-HSA-379716Reactome: R-HSA-6782315 |
| Cellular Localisation | CytoplasmCytosolMembranePeripheral Membrane ProteinTranslocates From Cytosol To Membranes Upon Phosphorylation At Ser-999 |
| Alternative Antibody Names | Anti-Bifunctional Glutamate/Proline--Trna Ligase antibodyAnti-Bifunctional Aminoacyl-Trna Synthetase antibodyAnti-Cell Proliferation-Inducing Gene 32 Protein antibodyAnti-Glutamatyl-Prolyl-Trna Synthetase Includes - Glutamate--Trna Ligase antibodyAnti-Glutamyl-Trna Synthetase antibodyAnti-Glurs - Proline--Trna Ligase antibodyAnti-Prolyl-Trna Synthetase antibodyAnti-EPRS1 antibodyAnti-EPRS antibodyAnti-GLNS antibodyAnti-PARS antibodyAnti-QARS antibodyAnti-QPRS antibodyAnti-PIG32 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
