• Western blot analysis of extracts of mouse liver, using EPHB4 antibody (STJ23550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EPHB4 antibody (300-540) (STJ23550)

SKU:
STJ23550

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPHB4 (300-540) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHB4
Gene ID: 2050
Uniprot ID: EPHB4_HUMAN
Immunogen Region: 300-540
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-540 of human EPHB4 (NP_004435.3).
Immunogen Sequence: AVCQCRVGYFRARTDPRGAP CTTPPSAPRSVVSRLNGSSL HLEWSAPLESGGREDLTYAL RCRECRPGGSCAPCGGDLTF DPGPRDLVEPWVVVRGLRPD FTYTFEVTALNGVSSLATGP VPFEPVNVTTDREVPPAVSD IRVTRSSPSSLSLAWAVPRA PSGAVLDYEVKYHEKGAEGP SSVRFLKTSENRAELRGLKR GASYLVQVRARSEAGYGPFG QEHHSQTQLDESEGWREQL
Tissue Specificity Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
Post Translational Modifications Phosphorylated.autophosphorylation is stimulated by EFNB2.
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
Protein Name Ephrin Type-B Receptor 4
Hepatoma Transmembrane Kinase
Tyrosine-Protein Kinase Tyro11
Database Links Reactome: R-HSA-2682334
Reactome: R-HSA-3928662
Reactome: R-HSA-3928664
Reactome: R-HSA-3928665
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Ephrin Type-B Receptor 4 antibody
Anti-Hepatoma Transmembrane Kinase antibody
Anti-Tyrosine-Protein Kinase Tyro11 antibody
Anti-EPHB4 antibody
Anti-HTK antibody
Anti-MYK1 antibody
Anti-TYRO11 antibody

Information sourced from Uniprot.org

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