Anti-EPHB4 antibody (300-540) (STJ23550)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EPHB4 (300-540) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EPHB4 |
| Gene ID | 2050 |
| Uniprot ID | EPHB4_HUMAN |
| Immunogen Region | 300-540 |
| Immunogen Sequence | AVCQCRVGYFRARTDPRGAP CTTPPSAPRSVVSRLNGSSL HLEWSAPLESGGREDLTYAL RCRECRPGGSCAPCGGDLTF DPGPRDLVEPWVVVRGLRPD FTYTFEVTALNGVSSLATGP VPFEPVNVTTDREVPPAVSD IRVTRSSPSSLSLAWAVPRA PSGAVLDYEVKYHEKGAEGP SSVRFLKTSENRAELRGLKR GASYLVQVRARSEAGYGPFG QEHHSQTQLDESEGWREQL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 300-540 of human EPHB4 (NP_004435.3). |
Additional Info
| Tissue Specificity | Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix. |
| Post Translational Modifications | Phosphorylated.autophosphorylation is stimulated by EFNB2. |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. |
| Protein Name | Ephrin Type-B Receptor 4Hepatoma Transmembrane KinaseTyrosine-Protein Kinase Tyro11 |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928662Reactome: R-HSA-3928664Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Ephrin Type-B Receptor 4 antibodyAnti-Hepatoma Transmembrane Kinase antibodyAnti-Tyrosine-Protein Kinase Tyro11 antibodyAnti-EPHB4 antibodyAnti-HTK antibodyAnti-MYK1 antibodyAnti-TYRO11 antibody |
Information sourced from Uniprot.org