Anti-Epcam antibody (24-266 aa) (STJ11105178)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Epcam (24-266 aa) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Epcam |
| Gene ID | 17075 |
| Uniprot ID | EPCAM_MOUSE |
| Immunogen Region | 24-266 aa |
| Immunogen Sequence | QRDCVCDNYKLATSCSLNEY GECQCTSYGTQNTVICSKLA SKCLAMKAEMTHSKSGRRIK PEGAIQNNDGLYDPDCDEQG LFKAKQCNGTATCWCVNTAG VRRTDKDTEITCSERVRTYW IIIELKHKERESPYDHQSLQ TALQEAFTSRYKLNQKFIKN IMYENNVITIDLMQNSSQKT QDDVDIADVAYYFEKDVKGE SLFHSSKSMDLRVNGEPLDL DPGQTLIYYVDEKAPEFSM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 24-266 of mouse Epcam. (NP_032558.2). |
Additional Info
| Post Translational Modifications | Glycosylation at Asn-198 is crucial for protein stability. |
| Function | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Protein Name | Epithelial Cell Adhesion MoleculeEp-CamEpithelial Glycoprotein 314Egp314Megp314Protein 289aTumor-Associated Calcium Signal Transducer 1Cd Antigen Cd326 |
| Database Links | Reactome: R-MMU-202733 |
| Cellular Localisation | Lateral Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionTight JunctionColocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction |
| Alternative Antibody Names | Anti-Epithelial Cell Adhesion Molecule antibodyAnti-Ep-Cam antibodyAnti-Epithelial Glycoprotein 314 antibodyAnti-Egp314 antibodyAnti-Megp314 antibodyAnti-Protein 289a antibodyAnti-Tumor-Associated Calcium Signal Transducer 1 antibodyAnti-Cd Antigen Cd326 antibodyAnti-Epcam antibodyAnti-Tacstd1 antibody |
Information sourced from Uniprot.org