| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC/IF/FC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-EndoPDI/TXNDC5 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 50kDa band observed in lysates of cell lines HEK293, A549, HeLa and HepG2 (calculated MW of 47.6kDa according to NP_110437.2). This molecular weight is routinely observed by oth |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | TXNDC5 |
| Gene ID: | 81567 |
| Uniprot ID: | TXND5_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_110437.2; NP_001139021.1 |
| Specificity: | This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2; NP_001139021.1. |
| Immunogen Sequence: | SLHRFVLSQAKDEL |
| Protein Name | Thioredoxin Domain-Containing Protein 5Endoplasmic Reticulum Resident Protein 46Er Protein 46Erp46Thioredoxin-Like Protein P46 |
| Database Links | Reactome: R-HSA-432720Reactome: R-HSA-432722Reactome: R-HSA-6798695 |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Thioredoxin Domain-Containing Protein 5 antibodyAnti-Endoplasmic Reticulum Resident Protein 46 antibodyAnti-Er Protein 46 antibodyAnti-Erp46 antibodyAnti-Thioredoxin-Like Protein P46 antibodyAnti-TXNDC5 antibodyAnti-TLP46 antibodyAnti-UNQ364 antibodyAnti-PRO700 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and TXNDC5 knockout (KO) 293T cells, using [KO Validated] TXNDC5 Rabbit polyclonal antibody (STJ11100807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89743/358218/STJ11100807_1__82919.1713122544.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TXNDC5 knockout (KO) 293T cells, using [KO Validated] TXNDC5 Rabbit polyclonal antibody (STJ11100807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and TXNDC5 knockout (KO) 293T cells, using [KO Validated] TXNDC5 Rabbit polyclonal antibody (STJ11100806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89742/358217/STJ11100806_1__90465.1713122542.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TXNDC5 knockout (KO) 293T cells, using [KO Validated] TXNDC5 Rabbit polyclonal antibody (STJ11100806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
