• STJ70588 staining (0. 1µg/ml) of HEK293 (A) , A549 (B) , HeLa (C) and (0. 01ug/ml) of HepG2 (D) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70588 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70588 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70588 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-EndoPDI/TXNDC5 antibody (C-Term) (STJ70588)

SKU:
STJ70588-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EndoPDI/TXNDC5 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.1µg/ml
IHC-3.75µg/ml
IF-Strong expression of the protein seen in the endoplasmic reticulum of U2OS and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TXNDC5
Gene ID: 81567
Uniprot ID: TXND5_HUMAN
Immunogen Region: C-Term
Accession Number: NP_110437.2; NP_001139021.1
Specificity: This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2; NP_001139021.1.
Immunogen Sequence: SLHRFVLSQAKDEL
Protein Name Thioredoxin Domain-Containing Protein 5
Endoplasmic Reticulum Resident Protein 46
Er Protein 46
Erp46
Thioredoxin-Like Protein P46
Database Links Reactome: R-HSA-432720
Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Thioredoxin Domain-Containing Protein 5 antibody
Anti-Endoplasmic Reticulum Resident Protein 46 antibody
Anti-Er Protein 46 antibody
Anti-Erp46 antibody
Anti-Thioredoxin-Like Protein P46 antibody
Anti-TXNDC5 antibody
Anti-TLP46 antibody
Anti-UNQ364 antibody
Anti-PRO700 antibody

Information sourced from Uniprot.org

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