Anti-EML3 antibody (300-530) (STJ11101449)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EML3 (300-530) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EML3 |
| Gene ID | 256364 |
| Uniprot ID | EMAL3_HUMAN |
| Immunogen Region | 300-530 |
| Immunogen Sequence | HYRGHTDCVRCLAVHPDGVR VASGQTAGVDKDGKPLQPVV HIWDSETLLKLQEIGLGAFE RGVGALAFSAADQGAFLCVV DDSNEHMLSVWDCSRGMKLA EIKSTNDSVLAVGFNPRDSS CIVTSGKSHVHFWNWSGGVG VPGNGTLTRKQGVFGKYKKP KFIPCFVFLPDGDILTGDSE GNILTWGRSPSDSKTPGRGG AKETYGIVAQAHAHEGSIFA LCLRRDGTVLS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 300-530 of human EML3 (NP_694997.2). |
Additional Info
| Post Translational Modifications | Phosphorylation at Thr-881 during mitosis is required for interaction with TUBG1, HAUS1, HAUS2, HAUS3, HAUS4, HAUS5, HAUS6, HAUS7 and HAUS8 and their recruitment to spindle microtubules. |
| Function | Regulates mitotic spindle assembly, microtubule (MT)-kinetochore attachment and chromosome separation via recruitment of HAUS augmin-like complex and TUBG1 to the existing MTs and promoting MT-based MT nucleation. Required for proper alignnment of chromosomes during metaphase. |
| Protein Name | Echinoderm Microtubule-Associated Protein-Like 3Emap-3 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonNucleusMidbodySpindleLocalizes To Microtubules Throughout All Mitotic Stages And Localizes To The Midbody During Cytokinesis |
| Alternative Antibody Names | Anti-Echinoderm Microtubule-Associated Protein-Like 3 antibodyAnti-Emap-3 antibodyAnti-EML3 antibody |
Information sourced from Uniprot.org