Anti-EMD antibody (1-220) (STJ23533)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EMD (1-220) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:20-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EMD |
| Gene ID | 2010 |
| Uniprot ID | EMD_HUMAN |
| Immunogen Region | 1-220 |
| Immunogen Sequence | MDNYADLSDTELTTLLRRYN IPHGPVVGSTRRLYEKKIFE YETQRRRLSPPSSSAASSYS FSDLNSTRGDADMYDLPKKE DALLYQSKGYNDDYYEESYF TTRTYGEPESAGPSRAVRQS VTSFPDADAFHHQVHDDDLL SSSEEECKDRERPMYGRDSA YQSITHYRPVSASRSSLDLS YYPTSSSTSFMSSSSSSSSW LTRRAIRPENRAPGAGLGQD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Emerin/Emerin/EMD (NP_000108.1). |
Additional Info
| Tissue Specificity | Skeletal muscle, heart, colon, testis, ovary and pancreas. |
| Post Translational Modifications | Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle. |
| Function | Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Required for proper localization of non-farnesylated prelamin-A/C. Together with NEMP1, contributes to nuclear envelope stiffness in germ cells. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. |
| Protein Name | Emerin |
| Database Links | Reactome: R-HSA-2980766Reactome: R-HSA-2995383Reactome: R-HSA-4419969Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408Reactome: R-HSA-9013423Reactome: R-HSA-9609523 |
| Cellular Localisation | Nucleus Inner MembraneSingle-Pass Membrane ProteinNucleoplasmic SideNucleus Outer MembraneColocalized With Banf1 At The Central Region Of The Assembling Nuclear RimNear Spindle-Attachment SitesThe Accumulation Of Different Intermediates Of Prelamin-A/C (Non-Farnesylated Or Carboxymethylated Farnesylated Prelamin-A/C) In Fibroblasts Modify Its Localization In The Nucleus |
| Alternative Antibody Names | Anti-Emerin antibodyAnti-EMD antibodyAnti-EDMD antibodyAnti-STA antibody |
Information sourced from Uniprot.org