Anti-ELF3 antibody (1-280) (STJ28454)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ELF3 (1-280) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ELF3 |
| Gene ID | 1999 |
| Uniprot ID | ELF3_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MAATCEISNIFSNYFSAMYS SEDSTLASVPPAATFGADDL VLTLSNPQMSLEGTEKASWL GEQPQFWSKTQVLDWISYQV EKNKYDASAIDFSRCDMDGA TLCNCALEELRLVFGPLGDQ LHAQLRDLTSSSSDELSWII ELLEKDGMAFQEALDPGPFD QGSPFAQELLDDGQQASPYH PGSCGAGAPSPGSSDVSTAG TGASRSSHSSDSGGSDVDLD PTDGKLFPSDGFRDCKKGD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ESE1/ESE1/ELF3 (NP_004424.3). |
Additional Info
| Tissue Specificity | Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin. |
| Function | Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGAAT. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development. |
| Protein Name | Ets-Related Transcription Factor Elf-3E74-Like Factor 3Epithelial-Restricted With Serine BoxEpithelium-Restricted Ets Protein EsxEpithelium-Specific Ets Transcription Factor 1Ese-1 |
| Database Links | Reactome: R-HSA-1912408 |
| Cellular Localisation | CytoplasmNucleusLocalizes To The Cytoplasm Where It Has Been Shown To Transform Mcf-12a Mammary Epithelial Cells Via A Novel Cytoplasmic MechanismAlso Transiently Expressed And Localized To The Nucleus Where It Induces Apoptosis In Non-Transformed Breast Epithelial Cells Mcf-10a And Mcf-12a Via A Transcription-Dependent Mechanism |
| Alternative Antibody Names | Anti-Ets-Related Transcription Factor Elf-3 antibodyAnti-E74-Like Factor 3 antibodyAnti-Epithelial-Restricted With Serine Box antibodyAnti-Epithelium-Restricted Ets Protein Esx antibodyAnti-Epithelium-Specific Ets Transcription Factor 1 antibodyAnti-Ese-1 antibodyAnti-ELF3 antibodyAnti-ERT antibodyAnti-ESX antibodyAnti-JEN antibody |
Information sourced from Uniprot.org