![Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90648/360589/STJ11101733_2__66065.1713125270.jpg?c=1 "Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90648/360590/STJ11101733_3__92590.1713125271.jpg?c=1 "Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90648/360591/STJ11101733_4__54322.1713125271.jpg?c=1 "Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human tonsil using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90648/360592/STJ11101733_5__41186.1713125272.jpg?c=1 "Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human tonsil using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human kidney using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90648/360593/STJ11101733_6__72804.1713125273.jpg?c=1 "Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human kidney using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90648/360594/STJ11101733_7__70782.1713125274.jpg?c=1 "Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90648/360595/STJ11101733_8__10677.1713125276.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90648/360596/STJ11101733_9__05625.1713125276.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-ELAVL1 antibody (1-100) [S3MR] (STJ11101733)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal HuR/ELAVL1 (1-100) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S3MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:200-1:2000IF/ICC:1:100-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your s |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ELAVL1 |
| Gene ID | 1994 |
| Uniprot ID | ELAV1_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MSNGYEDHMAEDCRGDIGRT NLIVNYLPQNMTQDELRSLF SSIGEVESAKLIRDKVAGHS LGYGFVNYVTAKDAERAINT LNGLRLQSKTIKVSYARPSS |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717). |
Additional Info
| Tissue Specificity | Ubiquitous. Detected in brain, liver, thymus and muscle. |
| Post Translational Modifications | Phosphorylated by MAPKAPK2. Phosphorylated by PRKCD. Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. |
| Function | RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation. Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUUAGUUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR. |
| Protein Name | Elav-Like Protein 1Hu-Antigen RHur |
| Database Links | Reactome: R-HSA-450520 |
| Cellular Localisation | CytoplasmNucleusStress GranuleP-BodyTranslocates Into The Cytoplasm Following Phosphorylation By Mapkapk2LikewisePhosphorylation By Prkcd Promotes Translocation From The Nucleus Into The CytoplasmWhere It Is Associated With Free And Cytoskeleton-Bound PolysomesLocalizes To The Stress Granules In The Presence Of Plekhn1 |
| Alternative Antibody Names | Anti-Elav-Like Protein 1 antibodyAnti-Hu-Antigen R antibodyAnti-Hur antibodyAnti-ELAVL1 antibodyAnti-HUR antibody |
Information sourced from Uniprot.org