• Immunohistochemistry analysis of paraffin-embedded mouse kidney using ELAC2 antibody (STJ29208) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ELAC2 antibody (STJ29208) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using ELAC2 antibody (STJ29208) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ELAC2 Antibody (STJ29208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ELAC2 antibody (1-270) (STJ29208)

SKU:
STJ29208

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ELAC2 (1-270) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELAC2
Gene ID: 60528
Uniprot ID: RNZ2_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ELAC2 (NP_776065.1).
Immunogen Sequence: MWALCSLLRSAAGRTMSQGR TISQAPARRERPRKDPLRHL RTREKRGPSGCSGGPNTVYL QVVAAGSRDSGAALYVFSEF NRYLFNCGEGVQRLMQEHKL KVARLDNIFLTRMHWSNVGG LSGMILTLKETGLPKCVLSG PPQLEKYLEAIKIFSGPLKG IELAVRPHSAPEYEDETMTV YQIPIHSEQRRGKHQPWQSP ERPLSRLSPERSSDSESNEN EPHLPHGVSQRRGVRDSSL
Tissue Specificity Widely expressed. Highly expressed in heart, placenta, liver, skeletal muscle, kidney, pancreas, testis and ovary. Weakly expressed in brain, lung, spleen, thymus, prostate, small intestine, colon and leukocytes.
Function Zinc phosphodiesterase, which displays mitochondrial tRNA 3'-processing endonuclease activity. Involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly.
Protein Name Zinc Phosphodiesterase Elac Protein 2
Elac Homolog Protein 2
Heredity Prostate Cancer Protein 2
Ribonuclease Z 2
Rnase Z 2
Trna 3 Endonuclease 2
Trnase Z 2
Database Links Reactome: R-HSA-6784531
Reactome: R-HSA-6785470
Reactome: R-HSA-8868766
Reactome: R-HSA-9708296
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Mitochondrion Nucleoid
Nucleus
Mainly Mitochondrial
Alternative Antibody Names Anti-Zinc Phosphodiesterase Elac Protein 2 antibody
Anti-Elac Homolog Protein 2 antibody
Anti-Heredity Prostate Cancer Protein 2 antibody
Anti-Ribonuclease Z 2 antibody
Anti-Rnase Z 2 antibody
Anti-Trna 3 Endonuclease 2 antibody
Anti-Trnase Z 2 antibody
Anti-ELAC2 antibody
Anti-HPC2 antibody

Information sourced from Uniprot.org

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