• Western blot analysis of lysate from HUVEC cells, uisng eIF5A2 antibody.
  • Western blot analysis of various cells using eIF5A2 Polyclonal Antibody diluted at 1:1000

Anti-EIF5A2 antibody (78-127 aa) (STJ92885)

SKU:
STJ92885

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Eukaryotic translation initiation factor 5A-2 (78-127 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF5A2
Gene ID: 56648
Uniprot ID: IF5A2_HUMAN
Immunogen Region: 78-127 aa
Specificity: eIF5A2 Polyclonal Antibody detects endogenous levels of eIF5A2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human eIF5A2 at the amino acid range 78-127
Post Translational Modifications Lys-50 undergoes hypusination, a unique post-translational modification that consists in the addition of a butylamino group from spermidine to lysine side chain and leads to the formation of a hypusine residue. eIF-5As are the only known proteins to undergo this modification, which is essential for their function.
Function Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step. Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity.
Protein Name Eukaryotic Translation Initiation Factor 5a-2
Eif-5a-2
Eif-5a2
Eukaryotic Initiation Factor 5a Isoform 2
Database Links Reactome: R-HSA-204626
Cellular Localisation Cytoplasm
Nucleus
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Hypusine Modification Promotes The Nuclear Export And Cytoplasmic Localization And There Was A Dynamic Shift In The Localization From Predominantly Cytoplasmic To Primarily Nuclear Under Apoptotic Inducing Conditions
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 5a-2 antibody
Anti-Eif-5a-2 antibody
Anti-Eif-5a2 antibody
Anti-Eukaryotic Initiation Factor 5a Isoform 2 antibody
Anti-EIF5A2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance