• Western blot analysis of lysates from A549 and COS7 cells, using EIF2AK1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from COLO205 cells using EIF2AK1 antibody.
  • Western blot analysis of various cells using HRI Polyclonal Antibody

Anti-EIF2AK1 antibody (571-620 aa) (STJ93592)

SKU:
STJ93592

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Eukaryotic translation initiation factor 2-alpha kinase 1 (571-620 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:100-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2AK1
Gene ID: 27102
Uniprot ID: E2AK1_HUMAN
Immunogen Region: 571-620 aa
Specificity: HRI Polyclonal Antibody detects endogenous levels of HRI protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human EIF2AK1 at the amino acid range 571-620
Function Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as heme deficiency, oxidative stress, osmotic shock, mitochondrial dysfunction and heat shock. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming. Acts as a key sensor of heme-deficiency: in normal conditions, binds hemin via a cysteine thiolate and histidine nitrogenous coordination, leading to inhibit the protein kinase activity. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell: heme depletion relieves inhibition and stimulates kinase activity, activating the ISR. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. It thereby plays an essential protective role for RBC survival in anemias of iron deficiency. Iron deficiency also triggers activation by full-length DELE1. Also activates the ISR in response to mitochondrial dysfunction: HRI/EIF2AK1 protein kinase activity is activated upon binding to the processed form of DELE1 (S-DELE1), thereby promoting the ATF4-mediated reprogramming.
Protein Name Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1
Heme-Controlled Repressor
Hcr
Heme-Regulated Eukaryotic Initiation Factor Eif-2-Alpha Kinase
Heme-Regulated Inhibitor
Hhri
Hemin-Sensitive Initiation Factor 2-Alpha Kinase
Database Links Reactome: R-HSA-9648895
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1 antibody
Anti-Heme-Controlled Repressor antibody
Anti-Hcr antibody
Anti-Heme-Regulated Eukaryotic Initiation Factor Eif-2-Alpha Kinase antibody
Anti-Heme-Regulated Inhibitor antibody
Anti-Hhri antibody
Anti-Hemin-Sensitive Initiation Factor 2-Alpha Kinase antibody
Anti-EIF2AK1 antibody
Anti-HRI antibody
Anti-KIAA1369 antibody
Anti-PRO1362 antibody

Information sourced from Uniprot.org

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