| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-EHMT2 (2-300) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S8MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EHMT2 |
| Gene ID: |
10919 |
| Uniprot ID: |
EHMT2_HUMAN |
| Immunogen Region: |
2-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 2-300 of human EHMT2 (Q96KQ7). |
| Immunogen Sequence: |
AAAAGAAAAAAAEGEAPAEM GALLLEKETRGATERVHGSL GDTPRSEETLPKATPDSLEP AGPSSPASVTVTVGDEGADT PVGATPLIGDESENLEGDGD LRGGRILLGHATKSFPSSPS KGGSCPSRAKMSMTGAGKSP PSVQSLAMRLLSMPGAQGAA AAGSEPPPATTSPEGQPKVH RARKTMSKPGNGQPPVPEKR PPEIQHFRMSDDVHSLGKVT SDLAKRRKLNSGGGLSEEL |
| Tissue Specificity | Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. |
| Post Translational Modifications | Methylated at Lys-185.automethylated. |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. Recruited to the promoters of target genes through interaction with transcriptional repressor MSX1, leading to the inhibition of myoblast differentiation via transcriptional repression of differentiation factors. |
| Protein Name | Histone-Lysine N-Methyltransferase Ehmt2Euchromatic Histone-Lysine N-Methyltransferase 2Hla-B-Associated Transcript 8Histone H3-K9 Methyltransferase 3H3-K9-Hmtase 3Lysine N-Methyltransferase 1cProtein G9a |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-3214841Reactome: R-HSA-427389Reactome: R-HSA-6804760Reactome: R-HSA-73762Reactome: R-HSA-8853884Reactome: R-HSA-8953750 |
| Cellular Localisation | NucleusChromosomeAssociates With Euchromatic RegionsDoes Not Associate With Heterochromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Ehmt2 antibodyAnti-Euchromatic Histone-Lysine N-Methyltransferase 2 antibodyAnti-Hla-B-Associated Transcript 8 antibodyAnti-Histone H3-K9 Methyltransferase 3 antibodyAnti-H3-K9-Hmtase 3 antibodyAnti-Lysine N-Methyltransferase 1c antibodyAnti-Protein G9a antibodyAnti-EHMT2 antibodyAnti-BAT8 antibodyAnti-C6orf30 antibodyAnti-G9A antibodyAnti-KMT1C antibodyAnti-NG36 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
