Anti-EHD1 antibody (N-Term) (STJ73648)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-EHD1 (N-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications. |
| Applications | Pep-ELISA/WB/IF/IHC/FC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in lysates of cell line NIH3T3 (calculated MW of 60.6kDa according to Mouse NP_0342491) Preliminary testing also showed a band at approx 60kDa in Human Lymph |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | EHD1 |
| Gene ID | 10938 |
| Uniprot ID | EHD1_HUMAN |
| Accession Number | NP_001269373.1; NP_001269374.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | FSWVSKDARRKKEPC |
Additional Info
| Function | ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis. |
| Protein Name | Eh Domain-Containing Protein 1Past Homolog 1Hpast1Testilin |
| Database Links | Reactome: R-HSA-983231 |
| Cellular Localisation | Recycling Endosome MembranePeripheral Membrane ProteinCytoplasmic SideEarly Endosome MembraneCell MembraneCell ProjectionCilium MembranePreferentially Associates With Tubular Recycling EndosomesColocalizes With Fer1l5 At Plasma Membrane In Myoblasts And MyotubesLocalizes To The Ciliary Pocket From Where The Cilium ProtrudesColocalizes With Bace1 In Tubulovesicular Cytoplasmic MembranesColocalizes With Bace1 And App Amyloid Beta Proteins In Hippocampal Mossy Fiber Terminals |
| Alternative Antibody Names | Anti-Eh Domain-Containing Protein 1 antibodyAnti-Past Homolog 1 antibodyAnti-Hpast1 antibodyAnti-Testilin antibodyAnti-EHD1 antibodyAnti-PAST antibodyAnti-PAST1 antibodyAnti-CDABP0131 antibody |
Information sourced from Uniprot.org