Anti-Eg5 antibody [4H3-1F12] (STJA0032343)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant human Eg5 protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-Eg5 for use in WB, ICC, IF and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4H3-1F12 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | KIF11 |
| Gene ID | 3832 |
| Uniprot ID | KIF11_HUMAN |
| Immunogen | Purified recombinant human Eg5 protein fragments expressed in E.coli. |
Additional Info
| Post Translational Modifications | Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis.phosphorylation is required for mitotic function. Ubiquitinated at Lys-1034 by UHRF1 via 'Lys-63'-linked ubiquitin chains, leading to interaction with spindle assembly factor TPX2, thereby ensuring accurate distribution to the spindles during metaphase. |
| Function | Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface. |
| Protein Name | Kinesin-Like Protein Kif11Kinesin-Like Protein 1Kinesin-Like Spindle Protein HkspKinesin-Related Motor Protein Eg5Thyroid Receptor-Interacting Protein 5Tr-Interacting Protein 5Trip-5 |
| Database Links | Reactome: R-HSA-2132295Reactome: R-HSA-6811434Reactome: R-HSA-983189 |
| Cellular Localisation | CytoplasmCytoskeletonSpindle Pole |
| Alternative Antibody Names | Anti-Kinesin-Like Protein Kif11 antibodyAnti-Kinesin-Like Protein 1 antibodyAnti-Kinesin-Like Spindle Protein Hksp antibodyAnti-Kinesin-Related Motor Protein Eg5 antibodyAnti-Thyroid Receptor-Interacting Protein 5 antibodyAnti-Tr-Interacting Protein 5 antibodyAnti-Trip-5 antibodyAnti-KIF11 antibodyAnti-EG5 antibodyAnti-KNSL1 antibodyAnti-TRIP5 antibody |
Information sourced from Uniprot.org