• STJ73333 (0. 3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73333 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-EEA1 antibody (821-835) (STJ73333)

SKU:
STJ73333-100

Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EEA1 (821-835) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IF-Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. 5µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EEA1
Gene ID: 8411
Uniprot ID: EEA1_HUMAN
Immunogen Region: 821-835
Accession Number: NP_003557.2
Immunogen Sequence: QETKIQHEELNNRIQ
Protein Name Early Endosome Antigen 1
Endosome-Associated Protein P162
Zinc Finger Fyve Domain-Containing Protein 2
Database Links Reactome: R-HSA-168138
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Early Endosome Antigen 1 antibody
Anti-Endosome-Associated Protein P162 antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 2 antibody
Anti-EEA1 antibody
Anti-ZFYVE2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance