• Western blot analysis of various lysates using EDC4 Rabbit polyclonal antibody (STJ117613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-EDC4 antibody (800-1100) (STJ117613)

SKU:
STJ117613

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EDC4 (800-1100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EDC4
Gene ID: 23644
Uniprot ID: EDC4_HUMAN
Immunogen Region: 800-1100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 800-1100 of human EDC4 (NP_055144.3).
Immunogen Sequence: GDGDRHNTPSLLEAALTQEA STPDSQVWPTAPDITRETCS TLAESPRNGLQEKHKSLAFH RPPYHLLQQRDSQDASAEQS DHDDEVASLASASGGFGTKV PAPRLPAKDWKTKGSPRTSP KLKRKSKKDDGDAAMGSRLT EHQVAEPPEDWPALIWQQQR ELAELRHSQEELLQRLCTQL EGLQSTVTGHVERALETRHE QEQRRLERALAEGQQRGGQL QEQLTQQLSQALSSAVAGR
Function In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).
Protein Name Enhancer Of Mrna-Decapping Protein 4
Autoantigen Ge-1
Autoantigen Rcd-8
Human Enhancer Of Decapping Large Subunit
Hedls
Database Links Reactome: R-HSA-430039
Cellular Localisation Cytoplasm
P-Body
Nucleus
Alternative Antibody Names Anti-Enhancer Of Mrna-Decapping Protein 4 antibody
Anti-Autoantigen Ge-1 antibody
Anti-Autoantigen Rcd-8 antibody
Anti-Human Enhancer Of Decapping Large Subunit antibody
Anti-Hedls antibody
Anti-EDC4 antibody
Anti-HEDLS antibody

Information sourced from Uniprot.org

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