Western blot analysis of extracts of various cell lines, using Dynein intermediate chain 1 antibody (STJ11103528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Dynein intermediate chain 1 antibody (1-200) [S8MR] (STJ11103528)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103528
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General Information

Short DescriptionRabbit monoclonal Dynein intermediate chain 1 (1-200) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS8MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolDNAI1
Gene ID27019
Uniprot IDDNAI1_HUMAN
Immunogen Region1-200
Immunogen SequenceMIPASAKAPHKQPHKQSISI GRGTRKRDEDSGTEVGEGTD EWAQSKATVRPPDQLELTDA ELKEEFTRILTANNPHAPQN IVRYSFKEGTYKPIGFVNQL AVHYTQVGNLIPKDSDEGRR QHYRDELVAGSQESVKVISE TGNLEEDEEPKELETEPGSQ TDVPAAGAAEKVTEEELMTP KQPKERKLTNQFNFSERASQ
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Dynein intermediate chain 1 (Q9UI46).

Additional Info

Tissue Specificity Expressed in respiratory ciliated cells (at protein level).
Function Part of the dynein complex of respiratory cilia.
Protein Name Dynein Axonemal Intermediate Chain 1
Axonemal Dynein Intermediate Chain 1
Database Links
Cellular Localisation Dynein Axonemal Particle
Cytoplasm
Cytoskeleton
Cilium Axoneme
Alternative Antibody Names Anti-Dynein Axonemal Intermediate Chain 1 antibody
Anti-Axonemal Dynein Intermediate Chain 1 antibody
Anti-DNAI1 antibody

Information sourced from Uniprot.org

Citations

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