• Western blot analysis of various lysates, using DVL1 antibody (STJ116411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using DVL1 antibody (STJ116411) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using DVL1 antibody (STJ116411) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 using DVL1 antibody (STJ116411) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 using DVL1 antibody (STJ116411) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DVL1 antibody (510-640) (STJ116411)

SKU:
STJ116411

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DVL1 (510-640) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DVL1
Gene ID: 1855
Uniprot ID: DVL1_HUMAN
Immunogen Region: 510-640
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 510-640 of human DVL1 (NP_004412.2).
Immunogen Sequence: GQGYPYQYPGPPPCFPPAYQ DPGFSYGSGSTGSQQSEGSK SSGSTRSSRRAPGREKERRA AGAGGSGSESDHTAPSGVGS SWRERPAGQLSRGSSPRSQA SATAPGLPPPHPTTKAYTVV GGPPGGPPVRE
Post Translational Modifications Ubiquitinated.undergoes both 'Lys-48'-linked ubiquitination, leading to its subsequent degradation by the ubiquitin-proteasome pathway, and 'Lys-63'-linked ubiquitination. The interaction with INVS is required for ubiquitination. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
Function Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).
Protein Name Segment Polarity Protein Dishevelled Homolog Dvl-1
Dishevelled-1
Dsh Homolog 1
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-201688
Reactome: R-HSA-4086400
Reactome: R-HSA-4641258
Reactome: R-HSA-4641262
Reactome: R-HSA-5368598
Reactome: R-HSA-5663220
Reactome: R-HSA-9673324
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytosol
Cytoplasmic Vesicle
Localizes At The Cell Membrane Upon Interaction With Frizzled Family Members
Alternative Antibody Names Anti-Segment Polarity Protein Dishevelled Homolog Dvl-1 antibody
Anti-Dishevelled-1 antibody
Anti-Dsh Homolog 1 antibody
Anti-DVL1 antibody

Information sourced from Uniprot.org

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