| Host: |
Mouse |
| Applications: |
FC/IF/IHC |
| Reactivity: |
All Eukaryotes |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Double strand DNA is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
AE-2 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG3k |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow cytometry (1-2 µg/million cells in 0, 1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of formal |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
SLC4A2 |
| Gene ID: |
6522 |
| Uniprot ID: |
B3A2_HUMAN |
| Immunogen: |
A hybrid (BALB/c x B6) mouse was immunized with nuclei of Raji Burkitt's lymphoma cells. |
| Tissue Specificity | Isoform A: Expressed in the liver, stomach, kidney, prostate, thyroid and rectum. Isoform B1: Expressed in the liver and kidney. Isoform B2: Expressed in the liver and kidney. |
| Function | Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane. Plays an important role in osteoclast differentiation and function. Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH. Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses. |
| Protein Name | Anion Exchange Protein 2Ae 2Anion Exchanger 2Non-Erythroid Band 3-Like ProteinBnd3lSolute Carrier Family 4 Member 2 |
| Database Links | Reactome: R-HSA-425381 |
| Cellular Localisation | Isoform A: Apical Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneIsoform B1: Apical Cell MembraneIsoform B2: Apical Cell Membrane |
| Alternative Antibody Names | Anti-Anion Exchange Protein 2 antibodyAnti-Ae 2 antibodyAnti-Anion Exchanger 2 antibodyAnti-Non-Erythroid Band 3-Like Protein antibodyAnti-Bnd3l antibodyAnti-Solute Carrier Family 4 Member 2 antibodyAnti-SLC4A2 antibodyAnti-AE2 antibodyAnti-EPB3L1 antibodyAnti-HKB3 antibodyAnti-MPB3L antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AES knockout (KO) HeLa cells, using [KO Validated] AES Rabbit polyclonal antibody (STJ11100820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89756/358232/STJ11100820_1__13212.1713122563.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AES knockout (KO) HeLa cells, using [KO Validated] AES Rabbit polyclonal antibody (STJ11100820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Anti-UACA antibody [AE-5] (STJ16101008)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/149724/302465/STJ16101008_1__59898.1646940649.jpg?c=1 "Anti-UACA antibody [AE-5] (STJ16101008)")