• Western blot analysis of extracts of various cell lines, using TdT antibody (STJ28010) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-DNTT antibody (260-509) (STJ28010)

SKU:
STJ28010

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNTT (260-509) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNTT
Gene ID: 1791
Uniprot ID: TDT_HUMAN
Immunogen Region: 260-509
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-509 of human TdT (NP_004079.3).
Immunogen Sequence: LKTSEKWFRMGFRTLSKVRS DKSLKFTRMQKAGFLYYEDL VSCVTRAEAEAVSVLVKEAV WAFLPDAFVTMTGGFRRGKK MGHDVDFLITSPGSTEDEEQ LLQKVMNLWEKKGLLLYYDL VESTFEKLRLPSRKVDALDH FQKCFLIFKLPRQRVDSDQS SWQEGKTWKAIRVDLVLCPY ERRAFALLGWTGSRQFERDL RRYATHERKMILDNHALYDK TKRIFLKAESEEEIFAHLG
Function Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Protein Name Dna Nucleotidylexotransferase
Terminal Addition Enzyme
Terminal Deoxynucleotidyltransferase
Terminal Transferase
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Nucleotidylexotransferase antibody
Anti-Terminal Addition Enzyme antibody
Anti-Terminal Deoxynucleotidyltransferase antibody
Anti-Terminal Transferase antibody
Anti-DNTT antibody
Anti-TDT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance