• Western blot analysis of lysates from mouse brain, using DMP1 Rabbit polyclonal antibody (STJ11103821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-DMP1 antibody (254-513) (STJ11103821)

SKU:
STJ11103821

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dentin Matrix Acidic Phosphoprotein 1 (254-513) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DMP1
Gene ID: 1758
Uniprot ID: DMP1_HUMAN
Immunogen Region: 254-513
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 254-513 of human DMP1 (NP_004398.1).
Immunogen Sequence: SGGSQLLEHPSRKIFRKSRI SEEDDRSELDDNNTMEEVKS DSTENSNSRDTGLSQPRRDS KGDSQEDSKENLSQEESQNV DGPSSESSQEANLSSQENSS ESQEEVVSESRGDNPDPTTS YVEDQEDSDSSEEDSSHTLS HSKSESREEQADSESSESLN FSEESPESPEDENSSSQEGL QSHSSSAESQSEESHSEEDD SDSQDSSRSKEDSNSTESKS SSEEDGQLKNIEIESRKLT
Tissue Specificity Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.
Post Translational Modifications Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif.
Function May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
Protein Name Dentin Matrix Acidic Phosphoprotein 1
Dmp-1
Dentin Matrix Protein 1
Database Links Reactome: R-HSA-3000178
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Nucleus
Cytoplasm
Secreted
Extracellular Space
Extracellular Matrix
In Proliferating Preosteoblasts It Is Nuclear
During Early Maturation Stage Is Cytoplasmic And In Mature Osteoblast Localizes In The Mineralized Matrix
Export From The Nucleus Of Differentiating Osteoblast Is Triggered By The Release Of Calcium From Intracellular Stores Followed By A Massive Influx Of This Pool Of Calcium Into The Nucleus
Alternative Antibody Names Anti-Dentin Matrix Acidic Phosphoprotein 1 antibody
Anti-Dmp-1 antibody
Anti-Dentin Matrix Protein 1 antibody
Anti-DMP1 antibody

Information sourced from Uniprot.org

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